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          N Antibody

          Datasheet
          • 貨號:
            CSB-RA33255A1GMY
          • 規格:
            ¥2640
          • 圖片:
            • Western Blot
              Positive WB detected in: His tag-tagged SARS-CoV-2 nucleocapsid recombinant protein from E. Coli (CSB-EP3325GMY)
              SARS-CoV-2 nucleocapsid antibody at 1:1000 (CSB-RA33255A1GMY)
              Secondary
              Peroxidase-Affinipure Goat Anti-Human IgG Fcγ Fragment Specific at 1/20000 dilution
              Predicted band size: 48 kDa
              Observed band size: 55 kDa
            • The Binding Activity of SARS-CoV-2-N Antibody with SARS-CoV-2-N
              Activity: Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-N at 2 μg/ml can bind SARS-CoV-2-N Antibody, the EC50 is 1.586 ng/ml.
            • In the Colloidal Gold Immunochromatography Assay detection system, the background of antibody (CSB-RA33255A1GMY) is clean, the detection limit can be as low as 125ng/ml (8.75ng/0.07ml), and the sensitivity is very good.
            • ELISA: Immobilize various types of SARS proteins at concentration of 2μg/ml on solid substrate, then react with SARS-CoV-2-N Antibody at concentration of 100μg/ml, 10μg/ml and 1μg/ml. It shows the SARS-CoV-2-N Antibody (CSB-RA33255A1GMY) is specific for SARS-CoV-2-N protein, without any cross-reactivity with NL63-CoV, HCoV-OC43, HCoV-229E or HCoV-HKU1.
            • The Binding Activity of N protein with N Recombinant Antibody. Activity: Measured by its binding ability in a functional ELISA. Immobilized N at 2 μg/ml can bind N Recombinant Antibody, the EC50 of N Recombinant Antibody.
            • The Binding Activity of N protein with N Recombinant Antibody. Activity: Measured by its binding ability in a functional ELISA. Immobilized N at 2 μg/ml can bind N Recombinant Antibody, the EC50 of N Recombinant Antibody.
            • The Binding Activity of N protein with N Recombinant Antibody. Activity: Measured by its binding ability in a functional ELISA. Immobilized N at 2 μg/ml can bind N Recombinant Antibody, the EC50 of N Recombinant Antibody.
          • 其他:

          產品詳情

          • 產品描述:

            Recombinant anti-SARS-CoV-2 Nucleoprotein (N protein) Mouse single-chain variable fragment (ScFv) is expressed from 293 cells (HEK293) with a human IgG1 Fc tag on C-terminal. The resulting product is the recombinant SARS-CoV-2 N monoclonal antibody. This N antibody is recommended for ELISA, WB, and GICA applications. It has undergone affinity-chromatography purification. And it can detect the endogenous content of human SARS-Cov-2 N protein.

            The SARS-CoV-2 N protein is an abundant RNA-binding protein. It contains two structured domains: the RNA binding domain and the oligomerization domain, which are separated by a long flexible linker. The separated domains allow the N protein to execute many functions in the viral lifecycle such as RNA replication, virion assembly, and immune system interference.

          • Uniprot No.:
          • 別名:
            Nucleocapsid protein, NC, protein N, N
          • 反應種屬:
            Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)
          • 免疫原:
            Recombinant Human Novel Coronavirus Nucleoprotein (N) (1-419aa) (CSB-EP3325GMY)
          • 免疫原種屬:
            Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)
          • 標記方式:
            Non-conjugated
          • 克隆類型:
            Monoclonal
          • 抗體亞型:
            Mouse scFv fusion with human IgG1 Fc
          • 純化方式:
            Affinity-chromatography
          • 克隆號:
            1A6
          • 濃度:
            It differs from different batches. Please contact us to confirm it.
          • 保存緩沖液:
            Preservative: 0.03% Proclin 300
            Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
          • 產品提供形式:
            Liquid
          • 應用范圍:
            ELISA, WB, GICA
          • 推薦稀釋比:
            Application Recommended Dilution
            ELISA 1:10000-1:50000
            WB 1:500-1:5000
            GICA 1:1000-1:10000
          • Protocols:
          • 儲存條件:
            Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
          • 貨期:
            Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.


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