N Antibody
Datasheet-
貨號：CSB-RA33255A1GMY
-
規格：￥2640
-
圖片：
-
Western Blot
Positive WB detected in: His tag-tagged SARS-CoV-2 nucleocapsid recombinant protein from E. Coli (CSB-EP3325GMY)
SARS-CoV-2 nucleocapsid antibody at 1:1000 (CSB-RA33255A1GMY)
Secondary
Peroxidase-Affinipure Goat Anti-Human IgG Fcγ Fragment Specific at 1/20000 dilution
Predicted band size: 48 kDa
Observed band size: 55 kDa -
The Binding Activity of SARS-CoV-2-N Antibody with SARS-CoV-2-N
Activity: Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-N at 2 μg/ml can bind SARS-CoV-2-N Antibody, the EC50 is 1.586 ng/ml. -
In the Colloidal Gold Immunochromatography Assay detection system, the background of antibody (CSB-RA33255A1GMY) is clean, the detection limit can be as low as 125ng/ml (8.75ng/0.07ml), and the sensitivity is very good. -
ELISA: Immobilize various types of SARS proteins at concentration of 2μg/ml on solid substrate, then react with SARS-CoV-2-N Antibody at concentration of 100μg/ml, 10μg/ml and 1μg/ml. It shows the SARS-CoV-2-N Antibody (CSB-RA33255A1GMY) is specific for SARS-CoV-2-N protein, without any cross-reactivity with NL63-CoV, HCoV-OC43, HCoV-229E or HCoV-HKU1. -
The Binding Activity of N protein with N Recombinant Antibody. Activity: Measured by its binding ability in a functional ELISA. Immobilized N at 2 μg/ml can bind N Recombinant Antibody, the EC50 of N Recombinant Antibody. -
The Binding Activity of N protein with N Recombinant Antibody. Activity: Measured by its binding ability in a functional ELISA. Immobilized N at 2 μg/ml can bind N Recombinant Antibody, the EC50 of N Recombinant Antibody. -
The Binding Activity of N protein with N Recombinant Antibody. Activity: Measured by its binding ability in a functional ELISA. Immobilized N at 2 μg/ml can bind N Recombinant Antibody, the EC50 of N Recombinant Antibody.
-
-
其他：
產品詳情
-
產品描述：
Recombinant anti-SARS-CoV-2 Nucleoprotein (N protein) Mouse single-chain variable fragment (ScFv) is expressed from 293 cells (HEK293) with a human IgG1 Fc tag on C-terminal. The resulting product is the recombinant SARS-CoV-2 N monoclonal antibody. This N antibody is recommended for ELISA, WB, and GICA applications. It has undergone affinity-chromatography purification. And it can detect the endogenous content of human SARS-Cov-2 N protein.
The SARS-CoV-2 N protein is an abundant RNA-binding protein. It contains two structured domains: the RNA binding domain and the oligomerization domain, which are separated by a long flexible linker. The separated domains allow the N protein to execute many functions in the viral lifecycle such as RNA replication, virion assembly, and immune system interference.
-
Uniprot No.：
-
別名：Nucleocapsid protein, NC, protein N, N
-
反應種屬：Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)
-
免疫原：Recombinant Human Novel Coronavirus Nucleoprotein (N) (1-419aa) (CSB-EP3325GMY)
-
免疫原種屬：Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)
-
標記方式：Non-conjugated
-
克隆類型：Monoclonal
-
抗體亞型：Mouse scFv fusion with human IgG1 Fc
-
純化方式：Affinity-chromatography
-
克隆號：1A6
-
濃度：It differs from different batches. Please contact us to confirm it.
-
保存緩沖液：Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 -
產品提供形式：Liquid
-
應用范圍：ELISA, WB, GICA
-
推薦稀釋比：
Application Recommended Dilution ELISA 1:10000-1:50000 WB 1:500-1:5000 GICA 1:1000-1:10000 -
Protocols：
-
儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
-
貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
